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Example Input Mode:
Partner Data
CBIS
Matrix
Example Data Type:
Secondary Screen (Kd)
Primary Screen(% control or competition)
If the input file contains Primary and Secondary Data, use:
Primary
Secondary
Assay Data File:
.txt or .csv format - download examples:
Bromodomain
Kd
Primary
To highlight spots, select a compound(s), click 'fill' and select entries.
Create Images for:
BMS-540215
Highlight These Spots (
fill
):
BMS-540215|ABL1-nonphosphorylated|1100
BMS-540215|ABL2|1800
BMS-540215|BLK|2000
BMS-540215|CSF1R|1900
BMS-540215|DDR1|160
BMS-540215|EPHA6|190
BMS-540215|EPHA7|1700
BMS-540215|EPHB6|1400
BMS-540215|FGFR1|99
BMS-540215|FGFR2|110
BMS-540215|FGFR3|160
BMS-540215|FGFR4|600
BMS-540215|FLT1|10
BMS-540215|FLT4|56
BMS-540215|HCK|2700
BMS-540215|KIT|36
BMS-540215|LCK|1100
BMS-540215|LOK|220
BMS-540215|MAP4K4|590
BMS-540215|MAP4K5|1400
BMS-540215|MEK5|3100
BMS-540215|MINK|310
BMS-540215|PDGFRA|11
BMS-540215|PDGFRB|50
BMS-540215|RET|390
BMS-540215|RIPK2|2900
BMS-540215|SLK|170
BMS-540215|SRC|1600
BMS-540215|STK33|3200
BMS-540215|STK35|26
BMS-540215|TIE1|1100
BMS-540215|TIE2|440
BMS-540215|TNIK|160
BMS-540215|VEGFR2|5
BMS-540215|YANK2|3900
BMS-540215|YSK4|500
BMS-540215|ABL1-nonphosphorylated
BMS-540215|ABL2
BMS-540215|BLK
BMS-540215|CSF1R
BMS-540215|DDR1
BMS-540215|EPHA6
BMS-540215|EPHA7
BMS-540215|EPHB6
BMS-540215|FGFR1
BMS-540215|FGFR2
BMS-540215|FGFR3
BMS-540215|FGFR4
BMS-540215|FLT1
BMS-540215|FLT4
BMS-540215|HCK
BMS-540215|KIT
BMS-540215|LCK
BMS-540215|LOK
BMS-540215|MAP4K4
BMS-540215|MAP4K5
BMS-540215|MEK5
BMS-540215|MINK
BMS-540215|PDGFRA
BMS-540215|PDGFRB
BMS-540215|RET
BMS-540215|RIPK2
BMS-540215|SLK
BMS-540215|SRC
BMS-540215|STK33
BMS-540215|STK35
BMS-540215|TIE1
BMS-540215|TIE2
BMS-540215|TNIK
BMS-540215|VEGFR2
BMS-540215|YANK2
BMS-540215|YSK4
BMS-540215|ABL1-nonphosphorylated|1100
BMS-540215|ABL2|1800
BMS-540215|BLK|2000
BMS-540215|CSF1R|1900
BMS-540215|DDR1|160
BMS-540215|EPHA6|190
BMS-540215|EPHA7|1700
BMS-540215|EPHB6|1400
BMS-540215|FGFR1|99
BMS-540215|FGFR2|110
BMS-540215|FGFR3|160
BMS-540215|FGFR4|600
BMS-540215|FLT1|10
BMS-540215|FLT4|56
BMS-540215|HCK|2700
BMS-540215|KIT|36
BMS-540215|LCK|1100
BMS-540215|LOK|220
BMS-540215|MAP4K4|590
BMS-540215|MAP4K5|1400
BMS-540215|MEK5|3100
BMS-540215|MINK|310
BMS-540215|PDGFRA|11
BMS-540215|PDGFRB|50
BMS-540215|RET|390
BMS-540215|RIPK2|2900
BMS-540215|SLK|170
BMS-540215|SRC|1600
BMS-540215|STK33|3200
BMS-540215|STK35|26
BMS-540215|TIE1|1100
BMS-540215|TIE2|440
BMS-540215|TNIK|160
BMS-540215|VEGFR2|5
BMS-540215|YANK2|3900
BMS-540215|YSK4|500
clear
Select Highlight spots color from Pallette:
(also used in table view)
Tree Image Options
Restore default options
Spot options:
IndianRed
PaleGreen
Gold
PowderBlue
Fill?
Outline?
Outline thickness:
1
2
3
4
5
Select Spots Color from Pallette:
(spot highlight color below)
micro
nano
Spot Size:
Scaling Factor:
.1
.2
.3
.4
.5
.6
.7
.8
.9
1
1.1
1.2
1.3
Area increase factor:
100%
90%
80%
70%
60%
50%
(per change of 10x Kd or 10% Competition)
Legend options:
Left align Text
Right align Text
Label options:
Compound Name?
Compound Name Font:
.5
1
1.5
2
Show Assay Panel information?
Show Panel Spots?
Append Concentration? (i.e., 'AB500@1000nm'; check before loading.)
Background Image:
Target labels?
Dark Tree Branch image?
Hide Lipid, Mutant, ATypical and Pathogen?
Spot options:
Spot Color transparency level:
none
low
medium-low
medium
high
no color
Spot Cutoff Value:
Kd (nM) (Blank for no cutoff)
Spot outline only?
Format:
JPEG(200KB per image)
Publication Quality(5MB image)
Create Publication Quality (5MB bitmap) Image for:
Select Compound
BMS-540215
size/quality
100
90
80
70
60
50
40
30
% of original
White Space Border:
Yes
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Preview and Highlighting
Highlight an assay by clicking on the spot or checking the box next to the Gene Symbol Name.
Click 'Update' to create the new images.
Compound To Highlight:
None
BMS-540215
Highlight Panel?
* WildType kinase assays appear in both the main TREEspot and Mutant kinase dendrogams.
Human Kinome image created by KINOMEscan.
2 images created. To download (copy and paste) all images to your local machine click Open/Browse Image Folder.
Images Saved To:
D:\Eurofins\PROD\Applications\FrontOffice_Discovery\StudyManagement\TreeSpot\HTSTools\TreeImages\164_154kg251t4cqbe2crxpixglj\
Open/Browse Image Folder
View Options:
(These options do not modify the images that have been created, only the view on this page)
BMS-540215
Legend
Show Selected
Size:
Select Size
small
medium
full size
Images per Row:
1
Legend Resize?
Hover the cursor to identify spots and click to highlighted it. To save an image click the Zoom/Download link.
Zoom/Download
Highlight
Locate an Assay
ABL1-nonphosphorylated
ABL1-nonphosphorylated
ABL2
BLK
CSF1R
DDR1
EPHA6
EPHA7
EPHB6
FGFR1
FGFR2
FGFR3
FGFR3
FGFR4
FLT1
FLT4
HCK
KIT
KIT
LCK
LOK
MAP4K4
MAP4K5
MINK
PDGFRA
PDGFRB
RET
RET
RIPK2
SLK
SRC
STK35
TIE1
TIE2
TNIK
VEGFR2
YSK4
Legend
Data Table View:
Show All Data
Only Selected Compounds/Trees
TREE
spot
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